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Howe, Christopher         Gene Cloning and Manipulation / Christopher Howe . – 2nd ed. . – Cambridge, UK : Cambridge University Press, 2007         ix, 266p.         The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms.         ISBN9780521817936 :         ISBN0521521055 :         ISBN9780521521055 :         1. Molecular cloning.         1. Cloning, Molecular – methods.         1. Escherichia coli – genetics.         1. Gene Library.         1. Transformation, Genetic.