TY - BOOK AU - Howe, Christopher TI - Gene Cloning and Manipulation SN - 9780521817936 U1 - 572.86/HOW/G PY - 2007/// CY - Cambridge, UK PB - Cambridge University Press KW - Molecular cloning KW - Cloning, Molecular KW - methods KW - Escherichia coli KW - genetics KW - Gene Library KW - Transformation, Genetic N1 - The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms ER -