Primrose, S. B.
Principles of Gene Manipulation and Genomics / S. B. Primrose and R. M. Twyman.
. – 7th ed.
. – Oxford : Blackwell Pub., 2014
xxii, 644 p. : ill.
Rev. ed. of: Principles of gene manipulation. 6th ed. 2001 and Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman. 3rd ed. 2003.
Gene manipulation in the post-genomics era -- Basic techniques -- Cutting and joining DNA molecules -- Basic biology of plasmid and phage vectors -- Cosmids, phasmids, and other advanced vectors -- Gene cloning strategies -- Sequencing genes and short stretches of DNA -- Changing genes : site-directed mutagenesis and protein engineering -- Bioinformatics -- Cloning in bacteria other than Escherichia coli -- Cloning in Saccharomyces cerevisiaea and other fungi -- Gene transfer to animal cells -- Genetic manipulation of animals -- Gene transfer to plants -- Advanced transgenic technology -- The organization and structure of genomes -- Mapping and sequencing genomes -- Comparative genomics -- Large-scale mutagenesis and interference -- Analysis of the transcriptome -- Proteomics I : expression analysis and characterization of proteins -- Proteomics II : analysis of protein structures -- Proteomics III : protein interactions -- Metabolomics and global biochemical networks -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci -- Applications of recombinant DNA technology.
ISBN97881726548392 :
1. Genetic engineering. 1. Genomics. 1. Gene mapping. 1. Nucleotide sequence. 1. Base Sequence. 1. Chromosome Mapping. 1. DNA, Recombinant. 1. Genomics.
I. Twyman, Richard M.